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The statistical analysis system (SPSS version 17.0) software was used to analyse all the data. The data were expressed as mean±standard error of mean (SEM) and the data were analysed using the Student’s t -test and one-way analysis of variance (ANOVA) followed by Tukeys post hoc multiple comparison test. Differences were considered statistically significant at P <0.05 for all the experiments used in this study. All treatments (six fishes in each treatment) were assayed in duplicate for each fish.

Haemoglobin contents and total erythrocyte counts significantly increased in group A, group B and group C at 30–60 days of feeding ( Table 2a and b , respectively). Total leucocyte count of C. catla significantly produced higher number of cells from 10days of feeding. Particularly, leucocyte count was higher in group B and group C than in group A from 40 to 60days of feeding with the experimental diet ( Cheap Buy Missoni Platform Espadrille Wedges Cheap Supply Discount Fake 100% Authentic Sale Online GYdtxv
).

Table 2 . Haematological values (mean ±SEM) of blood in fed with different concentrations (group A, group B and group C: 0.05%, 0.5% and 5%, respectively) of mixed diet between normal diet (control). Hemoglobin (Hb: g/dl), erythrocytes (RBC: 10 cellmm), leucocytes (WBC: 10mm) were obtained from 10, 20, 30, 40, 50 and 60days of feeding. Statistical differences (<0.05) among groups are indicated by asterisk (*).

Serum glucose level gradually increased in all experimental diet groups from 10 to 40 days of feeding ( Fig. 1a ). Glucose levels of group B (423±12.52 and 441.33±10.9) and group C (427.7±21.5 and 445.7±12.33) reached optimum at 30 and 40days of feeding, respectively. Then, the glucose level slightly reduced at 50 and 60days, although it was higher than that in the control and group A. In general, control and group A probably showed the same level of glucose.

Figure 1 . Biochemical values (mean±SEM) of blood serum in fed with different concentrations of mixed diet between normal diet (control). (a) Glucose (Hb: mg dL ), (b) cholesterol (mmol L), (c) total protein (gL), (d) albumin (gL), (e) globulin (gL), (f) albumin/globulin ratio (gL), (g) RNA/DNA ratio were obtained at 10, 20, 30, 40, 50 and 60days of feeding.

Serum cholesterol level of fish was significantly higher than that in the control in all experimental diet groups ( Fig. 1b ). Cholesterol level of group A was about similar to that of control at 30 and 40days of feeding (2.89±0.01 and 2.892±0.01), respectively. Cholesterol level was very lesser in control at 20 and 50days of feeding (2.702±0.04 and 2.72±0.01).

Born: 1981-09-12 AGE: 36 Veliko Tarnovo Bulgaria
Height 5'11" 180.34 cm Weight 203 lbs 92.08 kg
Association: Bushido Bulgaria
Class: Light Heavyweight
Wins 12 6 KO/TKO () 4 SUBMISSIONS () 2 DECISIONS ()
Losses 2 1 KO/TKO () 1 SUBMISSIONS () 0 DECISIONS ()
N/C 1

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